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AltoStar^® Purification Kit 1.5 RUO

Application

The AltoStar^® Purification Kit 1.5 uses magnetic particle technology and allows the isolation and purification of nucleic acids from biological specimens in conjunction with the AltoStar^® Automation System AM16 (Hamilton).

Specifications

Key features

DNA and RNA purification in parallel
All sample types in one run
Analyze up to four pathogens from a single sample

Sample types

The following sample types can be used with the AltoStar^® Purification Kit 1.5:

Whole blood

Serum

Plasma

Urine

Stool

Cerebrospinal fluid (CSF)

Swabs in transport medium

Can be used with

AltoStar^® Automation System AM16

Kit content

All reagents included in the AltoStar^® Purification Kit 1.5 are ready-to-use solutions and are shipped in two separate boxes:

Box 1 and Box 2. The boxes are shipped at room temperature.

Box 1 (with 6 different boxes inside) has to be stored at +15 °C to +30 °C and

Box 2 (with 6 trays inside) has to be stored at +2 °C to +8 °C upon receipt.

The AltoStar^® Purification Kit 1.5 contains reagents sufficient for 1,152 sample purifications with a 500 µl sample volume, or for 576 sample purifications with a 1,000 µl sample volume.

AltoStar^® Purification Kit 1.5 RUO – Content of Box 1 (with 6 different boxes inside)

Box 1 AltoStar® Purification Kit 1.5: The Lysis Buffer contains chaotropic salts (guanidine thiocyanate, octoxynol 9) to disrupt cells or virions chemically. It stabilizes nucleic acids and protects them against nucleases in solution (6 x container, Box 1/6).
Box 1 AltoStar® Purification Kit 1.5: The Wash Buffer 1 contains different salts and organic solvents (guanidine thiocyanate, ethanol) to remove proteins and other impurities (6 x container, Box 2/6).
Box 1 AltoStar® Purification Kit 1.5: The Wash Buffer 2 contains organic solvents (ethanol) to remove proteins and other impurities (6 x container, Box 3/6).
Box 1 AltoStar® Purification Kit 1.5: The Wash Buffer 3 contains different salts in order to purify the nucleic acids (6 x container, Box 4/6).
Box 1 AltoStar® Purification Kit 1.5: The Whole Blood Pretreatment Buffer contains surfactants and salts to stabilize and liquify whole blood samples for automated processing on the AltoStar® AM16 (8 x bottles, Box 5/6).
Box 1 AltoStar® Purification Kit 1.5: Container Re-Sealing Foil for the AltoStar® Purification Kit 1.5 Lysis Buffer and Wash Buffer 1, 2 and 3 (120 units, Box 6/6).

AltoStar^® Purification Kit 1.5 RUO – Content of Box 2 (6 trays with tubes inside)

Box 2 AltoStar® Purification Kit 1.5: This box contains 6 trays. The Enhancer stabilizes and protects nucleic acids against nuclease in solution. The Magnetic Beads are coated with a thin layer of silica to bind nucleic acids in solution. The magnetic characteristic allows the separation of beads from liquids in magnetic field. The Elution Buffer is a low salt buffer to elute the nucleic acids from the magnetic beads for subsequent analysis (one tray comprises 4 tubes Magnetic Beads, 2 tubes Elution Buffer and 4 tubes Enhancer).

Principle of method

The AltoStar^® Purification Kit 1.5 is intended for automated isolation and purification of RNA and DNA from specified biological specimens for in vitro diagnostic purposes in conjunction with the AltoStar^® AM16. The AltoStar^® Purification Kit 1.5 is based on magnetic bead technology, utilizing silica-coated magnetic particles, which can bind and release nucleic acids under specific conditions.

The purification procedure comprises 3 automated steps on the AltoStar^® AM16 (see figure).

Sample lysis and DNA/RNA binding

1. In the first step, nucleic acids are released by chemical and mechanical lysis under chaotropic high salt conditions. The conditions stabilize the nucleic acids in solution and enable their binding to the magnetic silica beads. The AltoStar^® Internal Control 1.5 is automatically added by the AltoStar^® AM16.

Purification