The AltoStar® Purification Kit 1.5 is intended for automated isolation and purification of RNA and DNA from specified human specimens for in vitro diagnostic purposes in conjunction with the AltoStar® AM16, the AltoStar® Internal Control 1.5, as well as with the altona Diagnostics kits and reagents, which are specified for use with the AltoStar® Purification Kit 1.5. The AltoStar® Purification Kit 1.5 is based on magnetic bead technology, utilizing silica-coated magnetic particles, which can bind and release nucleic acids under specific conditions.
The purification procedure comprises 3 automated steps on the AltoStar® AM16 (see figure).
1. In the first step, nucleic acids are released by chemical and mechanical lysis under chaotropic high salt conditions. The conditions stabilize the nucleic acids in solution and enable their binding to the magnetic silica beads. The AltoStar® Internal Control 1.5 is automatically added by the AltoStar® AM16.
2. In the following washing steps, different wash buffers are used to remove proteins and other impurities.
3. Finally, the nucleic acids are released from the magnetic beads with an elution buffer and transferred to the eluate plate.