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AltoStar® Purification Kit 1.5 CE

 

The AltoStar® Purification Kit 1.5 uses magnetic particle technology and is intended to be used for automated isolation and purification of nucleic acids from specified biological specimens for in vitro diagnostic purposes. The product is used in connection with the AltoStar® Automation System AM16, the AltoStar® Internal Control 1.5, as well as with the altona Diagnostics kits and reagents, which are specified for use with the AltoStar® Purification Kit 1.5.

The AltoStar® Purification Kit 1.5 is intended for use by professional users who are trained in molecular biological techniques.

AltoStar® Purification Kit 1.5 -Delivery of two boxes

Key Features

  • DNA and RNA Purification in parallel
  • All sample types in one run
  • Combine up to eight assays on a 96-well plate
  • Analyze up to four pathogens from a single sample
  • Scalable from 1-96, no minimum batch size
  • Sample carriers can be unloaded at any time after sample processing

Kit Content

 

All reagents included in the AltoStar® Purification Kit 1.5 are ready-to-use solutions and are shipped in two separate boxes: Box 1 and Box 2. The boxes are shipped at room temperature.

 

Box 1 (with 6 different boxes inside) has to be stored at +15 °C to +30 °C and

Box 2 (with 6 trays inside) has to be stored at +2 °C to +8 °C upon receipt.

 

The AltoStar® Purification Kit 1.5 contains reagents sufficient for 1152 sample purifications with a 500 µl sample volume, or for 576 sample purifications with a 1000 µl sample volume.

AltoStar® Purification Kit 1.5 - Content of Box 1 (with 6 different boxes inside)

AltoStar® Purification Kit 1.5 - Content of Box 2 (6 trays with tubes inside)

AltoStar® Purification Kit 1.5 CE

Order No.PK15-16
Sample purifications1152 (500 µl sample volume, low volume) or 576 (1000 µl sample volume, high volume)
Sample TypesHuman EDTA and citrate whole blood
Human serum
Human EDTA and citrate plasma
Human urine
Human stool
Swabs in viral transport medium
Human cerebrospinal fluid (CFS)
TransportationRoom temperature
For use withAltoStar® Automation System AM16
AltoStar® Internal Control 1.5
AltoStar® PCR kits
CFX96™ Deep Well Real-Time PCR Detection System-IVD

AltoStar® Purification Kit 1.5 CE

Instructions for use AltoStar® Purification Kit 1.5 CE

pdf
Release Date: 11/2018
Filesize: 1.88 MB

Instructions for use AltoStar® Purification Kit 1.5 RUO

pdf
Release Date: 11/2018
Filesize: 1.15 MB

Principle of Method

 

The AltoStar® Purification Kit 1.5 is intended for automated isolation and purification of RNA and DNA from specified biological specimens for in vitro diagnostic purposes in conjunction with the AltoStar® AM16, the AltoStar® Internal Control 1.5, was well as with the altona Diagnostics kits and reagents, which are specified for use with the AltoStar® Purification Kit 1.5. The AltoStar® Purification Kit 1.5 is based on magnetic bead technology, utilizing silica-coated magnetic particles, which can bind and release nucleic acids under specific conditions.

 

The purification procedure comprises 3 automated steps on the AltoStar® AM16 (see Fig.).

 

 

Sample lysis and DNA/RNA binding

1. In the first step nucleic acids are released by chemical and mechanical lysis under chaotropic high salt conditions. The conditions stabilize the nucleic acids in solution and enable their binding to the magnetic silica beads. The AltoStar® Internal Control 1.5 is automatically added by the AltoStar® AM16.

AltoStar® Purification Kit 1.5 CE

Konformitätserklärung/Declaration of Conformity/Déclaration de conformité - DE, EN, FR

pdf
Release Date: 21.12.2017
Filesize: 308.89 KB

Illustration of the AltoStar® purification procedure

pdf
Release Date: 11/2018
Filesize: 300.54 KB

Illustration de la procédure de purification AltoStar®

pdf
Date de sortie: 11/2018
Filesize: 300.54 KB