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AltoStar® Internal Control 1.5

 

The AltoStar® Internal Control 1.5 is intended to be used as a nucleic acid purification, amplification and detection control for in vitro diagnostic purposes. It is designed for use with the AltoStar® Purification Kit 1.5 and altona Diagnostics real-time PCR kits specified for use with the AltoStar® Internal Control 1.5.

 

The AltoStar® Internal Control 1.5 is intended for use by professional users trained in molecular biological techniques.

Key Features

  • One universal Internal Control (sample, extraction, PCR)
  • Contains a defined copy number of DNA and RNA template molecules with different sequences of artificial origin
  • Automatically added at the beginning of the nucleic acid purification procedure
  • Serves as a process control for purification and/or Real-time (RT-) PCR
  • Performance is verified in conjunction with each altona Diagnostics kit specified for use with the AltoStar® IC 1.5.
  • Lot checking and Expiry date checking

Kit Content

 

The AltoStar® Internal Control 1.5 box contains 24 tubes and is shipped on dry ice. After receipt the AltoStar®
Internal Control 1.5 must be stored at -25 °C to -15 °C.

 

Each IC (Internal Control) tube contains sufficient volume to perform 48 nucleic acid purifications with the AltoStar® Purification Kit 1.5.

AltoStar® Internal Control 1.5 CE

Order No.IC15-16
Sample purifications1152 (500 µl sample volume, low volume) or 576 (1000 µl sample volume, high volume)
Sample Typescompatible with all sample types that are specified
for use with the AltoStar® Purification Kit 1.5
TransportationDry ice
For use withAltoStar® Automation System AM16
AltoStar® Purification Kit 1.5
AltoStar® PCR kits
CFX96™ Deep Well Real-Time PCR Detection System-IVD

AltoStar® Internal Control 1.5 CE

Instructions for use AltoStar® Internal Control 1.5 CE

pdf
Release Date: 12/2017
Filesize: 2.25 MB

Mode d’emploi AltoStar® Internal Control 1.5 CE

pdf
Date de sortie: 12/2017
Filesize: 340.06 KB

Principle of Method

 

The AltoStar® Purification Kit 1.5 is intended for the automated isolation and purification of RNA and DNA from specified biological specimens for in vitro diagnostic purposes in conjunction with the AltoStar® AM16, the AltoStar® Internal Control 1.5 and altona Diagnostics kits and reagents specified for use with the AltoStar® Purification Kit 1.5. The AltoStar® Purification Kit 1.5 is based on magnetic bead technology, utilizing silica coated magnetic particles, which can bind and release nucleic acids under specific conditions.

 

The purification procedure comprises 3 automated steps on the AltoStar® AM16 (see Fig.).

 

 

Sample lysis and DNA/RNA binding

1. In the first step nucleic acids are released by chemical and mechanical lysis under chaotropic high salt conditions. The conditions stabilize the nucleic acids in solution and enable their binding to the magnetic silica beads. The AltoStar® Internal Control 1.5 is automatically added by the AltoStar® AM16.

AltoStar® Internal Control 1.5 CE

Konformitätserklärung/Declaration of Conformity/Déclaration de conformité - DE, EN, FR

pdf
Release Date: 21.12.2017
Filesize: 560.09 KB